FIG 9.

MLKL-dependent cell death is initiated upon inhibition of autophagy in HCMV-infected monocytes. (A and B) Human peripheral blood monocytes were mock or HCMV infected for 24 h, followed by either treatment with 0.5 μM necrosulfonamide (NSA) or cotreatment with NSA and Spautin-1 or vehicle control for an additional 24 h. Viability was measured by flow cytometry using PI and annexin staining. All experiments are representative of 4 independent experiments using different donors. (C and D) Pooled results from 4 independent blood donors were plotted by percentage of cells in either the “apoptotic” or “late dead” gates following treatment with Spautin-1 with or without NSA.