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. 2020 Oct 27;94(22):e01298-20. doi: 10.1128/JVI.01298-20

FIG 7.

FIG 7

Analysis of single-lysine-residue Zta mutants. (A) Schematic diagram of Zta lysine residues. (B) EBV-negative AdAH cells were transfected with 5 μg of a ubiquitin (His6-Ub) expression vector and 5 μg of the indicated Zta, Zta mutant, or control expression plasmids. Cells were lysed under denaturing conditions and extracts were purified over nickel-NTA beads. The purified material was assayed by Western blot analysis employing anti-Zta antibodies. (C) Comparable expression levels of Zta and its mutants were confirmed by Western blot analysis of whole-cell lysates.