FIG 9.

Loss of ubiquitination leads to deficit progression of Zta-mediated lytic replication. (A) EBV (Zta knockout)-infected 293 cells [EBV(Z K/O)-293] were transfected with wild-type or mutant Zta expression vectors or a control vector. Forty-eight hours posttransfection, cells were harvested and analyzed with each antibody shown. (B) EBV(Z K/O)-293 cells were transfected with wild-type or mutant Zta expression vectors or a control vector. Viral supernatants were harvested 5 days posttransfection and used to infect Raji cells. Since the virus from EBV(Z K/O)-293 cells contains a GFP expression cassette, the number of infected Raji cells was counted by FACS analysis. The infection index represents the percentage of GFP-positive cells (infected cells) relative to the number of GFP-positive cells observed following infection with supernatants from wild-type-Zta-transfected EBV(Z K/O)-293 cells. The averages and standard errors from the results of 3 independent infection assays are shown. Significance for differences between experimental groups were determined using Student’s t test using Prism 6 software, and P values of <0.05 were considered significant.