FIG 2.

Antigenic characterization of E2 designs using ELISA. Designs were cloned and expressed in the context of E1E2 as previously described (28) and tested for binding to a panel of HMAbs that target E2 antigenic domain A (CBH-4G and CBH-4B), B (HC-1), C (CBH-7), D (HC84.20, HC84.24, and HC84.26), and E (HC33.1 and HC33.4), at concentrations of 1 μg/ml and 5 μg/ml. Binding was tested to wild-type H77C E1E2 and compared with levels for the designs ΔHVR1 (E2 residues 384 to 407 deleted), ΔHVR1₄₁₁ (E2 residues 384 to 410 deleted), H445P, F627NT (F627N and V629T), R630NT (R630N and Y632T), K628NS (K628N and R630S), and Y632NS (Y632N and G634S). Asterisks denote designs that were tested in the context of ΔHVR1₄₁₁ (E2 residues 384 to 410 deleted) rather than full-length E1E2.