TABLE 1.
Sequence differences identified between parental G4 and pG4209 BAC by next-generation sequencinga
| Position | Nucleotide(s) in: |
Gene or location | Comments | Sanger sequencing | |
|---|---|---|---|---|---|
| G4 | G4209 | ||||
| 40711 | G | A | M32 | A266V | Mutation in G4209 |
| 42459 | G | T | M33 | A248S | Error in EU579859 |
| 42745 | A | C | M33 | Extends reading frame | Error in EU579859 |
| 93356/7 | CA | AC | Between m58 and M69 | No known effect | NDb |
| 93366 | C | A | Between m58 and M69 | No known effect | NDb |
| 93369 | A | C | Between m58 and M69 | No known effect | NDb |
| 110801 | T | A | M77 | F615Y | Error in EU579859 |
| 161922 | T | G | 5′ of stable intron | No known effect | Error in EU579859 |
| 161923/4 | Ex G | 5′ of stable intron | No known effect | Error in EU579859 | |
| 182390/1 | Ex TA | m124.1 | New start site (nt 182380)c | Error in EU579859 | |
| 187887 | Ex C | Mck-2 | Intron, extends m130 | Error in EU579859 | |
| 188422 | C | A | Putative 3′ UTR of sgg1 | Premature stop, sgg1.1 | Mutation in G4209 |
| 203305 | C | T | m144 | G156R | Error in EU579859 |
| 203746 | A | C | m144 | L9V | Error in EU579859 |
| 215377 | C | G | m155 | W32C | Error in EU579859 |
| 215436 | C | T | m155 | A13T | Error in EU579859 |
| 215518 | G | T | 5′ of m155 | No known effect | Error in EU579859 |
| 227940/1 | AC | CA | m168 | No known effectd | Error in EU579859 |
a
All nucleotide positions relate to the newly deposited sequence of G4 (EU579859.2). All putative sequence errors were confirmed or refuted by standard dye terminal sequencing; those shown to be in error in G4 have been corrected in the deposited sequence. Ex, extra; ND, no data.
b
Confirmation of changes in final Illumina sequencing of ARG4.
c
The new start site for m124.1 uses new G4 annotation.
d
Lack of m168 ORF identified in G4 following updated annotation using new sequence data.