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. 2020 Oct 27;8(2):e001493. doi: 10.1136/jitc-2020-001493

Figure 1.

Figure 1

Evaluation of mitogenic strength, T cell effector and Treg differentiation in response to SEG, SEI and SEG/SEI in vitro. CD4+ and CD8+ splenocytes from HLA-DQ8 tg, and C57BL/6 mice were incubated with SEA, SEB, SEG, SEI, (1 µg/mL) and SEG/SEI (0.5 µg/of each) for 72 hours and assayed for proliferation (A, B). CD4+ and CD8+T cells from HLA-DQ8 TG, and C57BL/6 mice were incubated with SEG, SEI (1 ug/mL) or SEG/SEI (0.5 µg/mL of each) for 72 hours and assayed for IFNγ (C, D) or granzyme and perforin (E, F). CD4 +T cells from HLA-DQ8 TG, and C57BL/6 mice were incubated with SEG, SEI, (1 µg/mL) or SEG/SEI (0.5 µg/mL of each) for 72 hours and FOXP3 expression was quantified (G). Data expressed as mean±SD. ANOVA with Tukey post test, n=3–5. *P<0.05, **p<0.01, ***p<0.001, ****p<0.001.