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. 2020 Oct 27;8(2):e001493. doi: 10.1136/jitc-2020-001493

Figure 6.

Figure 6

Vaccination with inactivated B16-F10 melanoma plus SEG/SEI in HLA-DQ8 tg mice induces long term survival. (A) HLA-DQ8 tg mice were treated with 1×106 irradiated (15 000 rads) B16-F10 melanoma cells ip on day −13 injected with SEG/SEI 50 µg of each ip on days −7 and −3 and challenged with 2.5×105 live B16-F10 cells IP day 0. Controls received live melanoma challenge alone on day 0 or irradiated B16-F10 cells on day −13 plus 2.5×105 live melanoma challenge on day 0 or SEG/SEI on days −7 and −3 plus 2.5×105 live melanoma cells.on day 0. HLA-DQ8 tg mice surviving >60 days (‘X’) were rechallenged with 2.5×105 live B16-F10 cells ip (X). (B) HLA-DQ8 Ttg mice were inoculated with 1×106 irradiated (15 000 rads) B16-F10 melanoma cells ip on day −13, injected with SEG/SEI, 50 µg of each, ip on days −7 and −3 and challenged with 2.5×105 live B16-F10 cells or Lewis lung carcinoma cells on day 0. Controls received 2.5×105 live B16-F10 cells or Lewis lung carcinoma cells alone on day 0. Kaplan-Meier curves with log-rank test, n=10. *P<0.05, ***p<0.001. Acronym ‘R’ denotes irradiated tumor cell vaccination on day −13 and ‘C’ indicates challenge with either B16-F10 melanoma or Lewis lung carcinoma tumor cells on day 0. ns, not significant.