Fig. 3.

Circ_0001421 could act as a sponge for miR-4677-3p. a The predicted binding sites between circ_0001421 and miR-4677-3p were shown. b The expression of miR-4677-3p in H1299 and A549 cells transfected with miR-NC or miR-4677-3p was detected by qRT-PCR. (C) Dual-luciferase reporter assay was performed to verify the relationship between circ_0001421 and miR-4677-3p in H1299 and A549 cells. d miR-4677-3p expression in tumor and adjacent normal tissues was examined by qRT-PCR. e qRT-PCR was used to determine miR-4677-3p expression in A549, H1299 and 16HBE cells. f The correlation between circ_0001421 and miR-4677-3p expression was analyzed in LC tissues by Pearson coefficient analysis. g miR-4677-3p expression in H1299 and A549 cells transfected with si-NC or si-circ_0001421 was measured by qRT-PCR. *P < 0.05