Fig. 5.

LPA induces in vivo mammary tumorigenicity via RAGE. a, b, c, d Representative images showing tumor foci on peritoneal wall of nude mice injected with control shRNA and RAGE treated MDA MB-231 and MCF-7 cells with and without LPA stimulation. Right, Quantification of number of tumor foci on peritoneal wall of nude mice injected with control shRNA and RAGE treated MDA MB-231 and MCF-7 cells with and without LPA stimulation (n = 4 for each group). e Representative images show Ki67 immunohistochemical staining of the tumor sections of nude mice injected with control shRNA and RAGE treated MDA MB-231 and MCF-7 cells with and without LPA stimulation and its quantitative representation. f Immunohistochemical analysis and quantifications of % area DAB in sections of p-PKB (Ser473) in tumor sections of control shRNA and RAGE shRNA MDA MB-231 and MCF-7 cells intraperitoneally injected nude mice with exogenous LPA treatment. For all experiments, data are means ± SD., n = 4. ***P ≤ 0.001