Figure 2.

Effects of STX‐0119 on STAT3 activation and fibrotic genes in mouse UUO kidneys. STX‐0119 was administered once daily for 3 days. The mice were sacrificed at day 3 after UUO surgery. Each gene was normalized by Gapdh mRNA. (a) Effects of STX‐0119 on p‐STAT3/total STAT3 in mouse UUO kidneys. p‐STAT3 and STAT3 protein expression levels were evaluated by AlphaLISA. (b–d) Effects of STX‐0119 on STAT3 downstream genes (Myc [b], Ccl12 [c], and Cxcl10 [d]) in mouse UUO kidneys. (e–i) Effects of STX‐0119 on fibrotic genes in mouse UUO kidneys. *p < .05, **p < .01, ***p < .001 versus sham; #p < .05, ##p < .01, ###p < .001 versus UUO + Vehicle; one‐way analysis of variance + Tukey's test. n.s., not significant. N = 4 for sham group and N = 9 for other groups. These results were replicated by three times at independent experiments