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. 2020 Sep 10;2(1):vdaa115. doi: 10.1093/noajnl/vdaa115

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© The Author(s) 2020. Published by Oxford University Press, the Society for Neuro-Oncology and the European Association of Neuro-Oncology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Cytoplasmic sequestration of bHLH TF. (A) Immunocytochemistry of LN229 dnE47-RFP and (B) LN229 E47-RFP. Confocal microphotograph, merged microphotographs (1) and 4′,6-diamidino-2-phenylindole (2), ID1 (3), and Phalloidin (4). RFP distribution in cells (5) (bar = 20 µm). (C) Increased cytoplasmic sequestration of ID1. Immunoblots with nuclear (Nuc.) and cytoplasmatic (Cyt.) protein fractions of LNZ308 RFP-dnE47 and RFP-E47; IkBα (cyt) and β-Tubulin (Nuc) as internal controls.