Figure 3.

CAGE analysis. (A) Promoter shifting analysis. Boxplots show consensus clusters with a shifting score ≥0.15. Significant increase of shifting promoters at timepoints 24, 48, and 72 h after Dox induction. Statistical testing was performed with the Mann–Whitney test: 24 h (P value: <.0001 (***)), 48 h (P value: .03 (*)), and 72 h (P value: .0004 (***)). Shifting promoters were associated with transcriptionally relevant genome annotation features “overlap start” (blue), “inside” (green), “downstream” (orange), “upstream” (violet). X-axis labels: +Dox = dnE47-RFP + Dox versus E47-RFP + Dox; −Dox = dnE47-RFP-Dox versus E47-RFP-Dox. (B and C) TF-binding enrichment analysis after 72 h of those consensus clusters with a shifting score ≥0.15 showed a time-related increase in TF activity (B), especially for class 1 (basic domains), class 2 (Zinc-coordinating DNA-binding domains), and class 3 (helix-turn-helix domains). An enrichment of specific TF-binding domains was observed for the E2F (C, blue bars) and Sp-family (C, red bars).