Table 7.

Assessment of the limits-of-detection (lod) applying DNA plasmids as positive control in quadruplicate

Positive control DNA copy number	Ct-values of the ARIES ORF-sequence				Ct-values of the ARIES N-gene				Ct-values of the ARIES the internal control (RNAse-P-gene)
	Run 1	Run 2	Run 3	Run 4	Run 1	Run 2	Run 3	Run 4	Run 1	Run 2	Run 3	Run 4
1.4 × 106	25.3	21.7	21.7	21.9	27.2	23.7	23.9	24.1	negative	negative	negative	negative
1.4 × 105	28.6	24.1	25.5	24.2	30.7	26.6	27.7	27.1	negative	negative	negative	negative
1.4 × 104	29.6	25.0	27.3	negative	31.5	30.1	30.4	32.5	negative	negative	negative	negative
1.4 × 103	32.3	negative	negative	negative	34.4	36.2	36.5	37.2	negative	negative	negative	negative
1.4 × 102	negative	negative	negative	negative	negative	negative	38.3	38.8	39.1	25.3	negative	negative
1.4 × 101	negative	negative	negative	negative	negative	negative	negative	negative	negative	25.7	26.4	26.8
1.4	negative	negative	negative	negative	negative	negative	negative	negative	negative	27.0	27.0	26.6
<1	negative	negative	negative	negative	negative	negative	negative	negative	negative	27.1	27.1	26.9
<1	negative	negative	negative	negative	negative	negative	negative	negative	negative	27.1	26.1	27.1

Ct = cycle threshold.