Figure 1.

A: PCR genotyping demonstrates the deletion of NK1R (Tacr1) and Mdr2 (Abcb4) in NK1R^−/−/Mdr2^−/− (Tacr1^−/−/Abcb4^−/−) mice. B: Immunofluorescence in liver sections demonstrates neurokinin 1 receptor (NK1R) immunoreactivity in cholangiocytes from wild-type (WT) and Mdr2^−/− mice, immunoreactivity that was absent in both NK1R^−/− and NK1R^−/−/Mdr2^−/− mice. C: By immunoblots, there is low expression of NK1R in cholangiocytes from WT mice, which increased in cholangiocytes from Mdr2^−/− mice; no expression for NK1R is observed in either NK1R^−/− or NK1R^−/−/Mdr2^−/− mice. The immunoblot was performed in cumulative preparations of isolated cholangiocytes from six mice. ∗P < 0.05 versus WT; ^†P < 0.05 versus Mdr2^−/− mice. Scale bars = 100 μm (B). Original magnification, ×40 (B). CK-19, cytokeratin-19; MW, molecular weight; TBP, tata-binding protein.