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. 2014 Oct 23;22(1):29–37. doi: 10.3727/096504014X14078436004987

Figure 4.

Figure 4

U0126 reversed the phenotype of TGF-β1-induced EMT in NSCLC cell lines. H1650 and H1299 cells were incubated with TGF-β1 (5 ng/ml) for 72 h, and then U0126 (10 µM) was added for another 48 h. (A) Morphological changes were examined in control cells (RP1640) and cells treated with TGF-β1 (5 ng/ml) and TGF-β1 and U0126. (B) The expression of ERK, p-ERK, FOXM1, E-cadherin, and vimentin was determined by Western blotting. (D) The migration capacity of H1650 and H1299 cells treated with TGF-β1 and U0126 was determined by wound-healing assays (**p < 0.01).