Fig 5. Mock PCR experiments for characterisation of printed resin interference with PCR.

(a) Schematic illustration of mock PCR experiments for low SVR incubated prints. Printed samples representative of consecutive post-processing steps including isopropanol (IPA) wash, UV curing and hot water baths were incubated into the PCR mixture and remained until the completion of the reaction, (b) Gel electrophoresis imaging of separated PCR amplicons from mock reactions: low SVR printed samples incubated in PCR mixture during PCR. Samples post-processed with high intensity UV light and hot water washes (bands 5–9) showed minimum interaction with DNA amplification, unlike the ones post-processed only with isopropanol washes and/or UV curing (bands 2–4) (S1A_raw_images in S1 File (DOI: 10.25405/data.ncl.12320501)).