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. Author manuscript; available in PMC: 2020 Oct 28.
Published in final edited form as: J Am Chem Soc. 2019 Oct 23;141(44):17517–17521. doi: 10.1021/jacs.9b10217

Figure 2.

Figure 2.

Characterization of the biosynthesis of the central C ring (in red). (a) In vivo reconstitution of dmaW, easC, easE, and easF in A. nidulans. (b) In vitro reconstitution of the activity of EasC. (c) Analysis of 18O-labeled 2 generated from EasC-reaction in 18O2 saturated buffer. LC-HRMS data: 18O-labeled chanoclavine-I 2 [M + H]+ m/z: calculated 259.1696, observed 259.1691. (d) Complete biosynthetic pathway from tryptophan to 5. See SI for reaction conditions.