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. 2020 Oct 16;16(10):e1009052. doi: 10.1371/journal.pgen.1009052
mNeonGreen amplification from
dg357 (forward) for donor TGTCAGCGTGCATTGAATGTTTGATTGCAGAAAATCCA
GCCGCTAAAGGAGGTGGCGGATCTGGAGGTGGAGGCTCTG GAGGAGGTGGATCTATGGTGTCGAAGGGAGAAG
mNeonGreen amplification from
dg357 (reverse) for donor
TATACAAAAAAACAGTATATACAATTTAGCATATGCTACTTGTAGAGTTCATCCATTC
mScarlet-I amplification from
pSEM90 (forward) for donor
TGTCAGCGTGCATTGAATGTTTGATTGCAGAAAATCCAGCCG CTAAAGGAGGTGGCGGATCTGGAGGTGGAGGCTCTGGAGGAGGTGGATCTATGGTCAGCAAGGGAGAGG
mScarlet-I amplification from
pSEM90 (reverse) for donor
TATACAAAAAAACAGTATATACAATTTAGCATATGCTACTTGTAGAGCTCGTCCATTCC
crRNA (reverse strand) AGCAUAUGUCACUUCGCUGC