Table 3.
Multivariate analysis of parameters associated with disease-free survival
| N (%) | 5-year DFS | TIL (H&E stained) |
CD8+ cell density |
|||||
|---|---|---|---|---|---|---|---|---|
| HR | 95% CI | p value | HR | 95% CI | p value | |||
| Sex | ||||||||
| Male | 61 (50) | 47% | 1.0 | Reference | 1.0 | Reference | ||
| Female | 61 (50) | 39% | 1.03 | 0.63–1.68 | 0.88 | 0.99 | 0.61–1.61 | 0.98 |
| Age | ||||||||
| ≤70 years | 56 (46) | 42% | 1.0 | Reference | 1.0 | Reference | ||
| >70 years | 66 (54) | 45% | 1.15 | 0.72–1.86 | 0.54 | 1.06 | 0.66–1.72 | 0.78 |
| Location of the tumor 1 | ||||||||
| Right side of the colon | 57 (47) | 44% | 1.0 | Reference | 1.0 | Reference | ||
| Left side of the colon | 65 (53) | 41% | 1.33 | 0.83–2.16 | 0.22 | 1.26 | 0.79–2.02 | 0.32 |
| Histologic type | ||||||||
| Well | 48 (39) | 43% | 1.0 | Reference | 1.0 | Reference | ||
| Moderate | 67 (55) | 40% | 1.01 | 0.61–1.68 | 0.96 | 1.03 | 0.63–1.17 | 0.89 |
| Poor | 2 (2) | 50% | 1.02 | 0.05–5.01 | 0.97 | 1.21 | 0.06–6.11 | 0.85 |
| Mucinous | 5 (4) | 75% | 1.32 | 0.38–3.51 | 0.62 | 1.66 | 0.46–4.68 | 0.39 |
| Adjuvant chemotherapy | ||||||||
| Yes | 63 (52) | 42% | 1.0 | Reference | 1.0 | Reference | ||
| No | 59 (48) | 45% | 0.93 | 0.58–1.48 | 0.77 | 0.95 | 0.60–1.52 | 0.85 |
| Invasive margin of tumor | ||||||||
| TIL (H-E stained) | ||||||||
| Positive | 95 (78) | 48% | 1.0 | Reference | ||||
| Negative | 27 (22) | 27% | 1.81 | 1.03–3.05 | 0.037 | |||
| Central part of tumor CD8+ | ||||||||
| Positive | 60 (49) | 51% | 1.0 | Reference | ||||
| Negative | 62 (51) | 34% | 1.76 | 1.07–2.93 | 0.023 | |||
TIL, tumor-infiltrating lymphocyte.
1
Right-sided colon cancers included tumors arising from the cecum, ascending colon, hepatic flexure, and transverse colon, while left-sided colon cancers included tumors arising from the descending colon and sigmoid colon.