Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (human) | PGP1 | Lee et al., 2009 | GM23338 | Male; mycoplasma-free |
| Cell line (human) | TNNT2-GFP | This paper | Derived from PGP1 cell line GM23338, mycoplasma-free | |
| Commercial assay or kit | Zero Blunt TOPO PCR Cloning Kit | ThermoFisher | K280002 | |
| Commercial assay or kit | Human Stem Cell Nucleofector Kit | Lonza | VPH-5022 | |
| Commercial assay or kit | Nextera XT Sample Preparation Kit | Illumina | FC-131–1096 | |
| Commercial assay or kit | Nextera DNA Sample Kit (ATAC-seq) | Illumina | FC-121–1030 | |
| Commercial assay or kit | Tru ChIP Chromatin Shearing Hit | Covaris | 520154 | |
| Commercial assay or kit | Chromium i7 Multiplex Kit | 10X Genomics | 1000073 | |
| Commercial assay or kit | Chromium Chip B Single-Cell Kit | 10X Genomics | 1000075 | |
| Sequence-based reagent | Guide RNAs | This paper |
GATA6 Exon 2 Guide 1: GAGCCCCTACTCGCCCTACG GATA6 Exon 2 Guide 2: GCCCCTACTCGCCCTACGTG GATA6 Exon 4 Guide 1: GGCGTTTCTGCGCCATAAGG |
|
| Sequence-based reagent | GATA6 sequencing primers | This paper | PCR Primers | GATA6 Exon 2 Sequencing Primer Left/Forward GACGTACCACCACCACCA GATA6 Exon 2 Sequencing Primer Right/Reverse CTTACCTGCACTGGGACCC GATA6 Exon 4 Sequencing Primer Left/Forward TGAATTCACGGAGACAGGCT GATA6 Exon 4 Sequencing Primer Right/Reverse TACAAGTGAGCAGAATACATGGCA |
| Sequence-based reagent | ATAC-seq amplification oligos | Buenrostro et al., 2015 | ||
| Recombinant DNA reagent | Cas9 plasmid | Addgene | PX459v2 | |
| Chemical compound, drug | WIN 18446 | Tocris | ||
| Antibody | rabbit mono-clonal Gata6 | Cell Signaling Technology | 5851S | 10 ug/ChIP |
| Antibody | rabbit mono-clonal Gata6 | Abcam | Ab175927 | 1:1000 dilution |
| Software, algorithm | RNA-seq pipeline: bcbio-nextgen | Chapman et al., 2020 | v.1.2.3 | Hg19 |
| Software, algorithm | R Package: DESEQ2 | Love et al., 2014 | v. 2.1.18.1 | |
| Software, algorithm | R Package: Seurat | Stuart et al., 2019 | v.3.0.0 | |
| Software, algorithm | R Package: ChIP-seeker | Yu et al., 2015 | v.1.14.1 | |
| Software, algorithm | HOMER | Heinz et al., 2010 | v4.10.3 |