Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Recombinant DNA reagent	HemK wt plasmid	DOI: 10.1126/science.aad0344	ID_ECBD_2409
Recombinant DNA reagent	HemK 4xA plasmid	DOI: 10.1126/science.aad0344
Recombinant DNA reagent	HemK looped plasmid	This paper		Methods: HemK constructs for PET and FPA; Supplementary file 1 - table 10
Recombinant DNA reagent	HemK 4xA FPA plasmid	This paper		Methods: HemK constructs for PET and FPA; Supplementary file 1 - table 11
Recombinant DNA reagent	HemK wt FPA plasmid	This paper		Methods: HemK constructs for PET and FPA; Supplementary file 1 - table 11
other	Purified E. coli in vitro translation system	DOI: 10.1073/pnas.92.6.1945; 10.1021/ja407511q; 10.1126/science.1229017; 10.1126/science.aad0344; 10.1016/S0076-6879 (07)30001–3
Chemical compound, drug	BODIPY FL NHS-Ester	Thermo Fisher Scientific	ID_D2184
Chemical compound, drug	ATTO 655-NHS-Ester	ATTO-Tec GmbH, Siegen, Germany	ID_AD 655–31
Software, algorithm	Prism GraphPad	GraphPad Software, La Jolla California USA, www.graphpad.com
Software, algorithm	KinTek Explorer, version 6.3	KinTek Global Kinetic Explorer V 6.3 https://kintekcorp.com/software
Software, algorithm	MicroTime 200 system	MicroTime 200 system, PicoQuant, Berlin, Germany
Software, algorithm	SymPhoTime 64 version 2.2	PicoQuant, Berlin, Germany