Fig. 5.

Effects of voltage-dependent channel block induced by p-CF₃ diEPP on ACh responses at the α7 nAChR. Responses evoked by coapplication of 30 μM p-CF₃ diEPP with 60 μM ACh at different holding potentials (millivolts). The ACh residual responses measured in the presence of p-CF₃ diEPP were 0.19 ± 0.05 at −90 mV (n = 5), 0.34 ± 0.07 at −70 mV (n = 5), 0.44 ± 0.05 at −50 mV (n = 7), and 0.59 ± 0.05 at −30 mV (n = 4) relative to 60 μM ACh control responses obtained at the same potentials. Averaged raw data traces showing the effects of holding potential on the inhibition of ACh currents in the presence of p-CF₃ diEPP at −90 and −50 mV are shown below the graph. The control traces were scaled to have the same amplitude, and the coapplication responses are relative to the controls.