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. 2020 Jun 9;17:59–70. doi: 10.2142/biophysico.BSJ-2020007

Figure 3.

Figure 3

The membrane current response of hWR-W163F in a standard extracellular/intracellular milieu. (A) Each sample trace was a current response to the double light pulses at each Vm. The symbols indicate the Ipeak/Iend of the first responses (blue filled/open diamonds) and the Ipeak/Iend of the second responses (red filled/open diamonds), respectively. Note the holding-current shift to the negative direction after the first light pulse (arrow). (B) I-V relations of the Ipeak; the first responses (blue symbols) and the second responses (red symbols). (C) The Iend was corrected for the change in the holding current (Iend') and expressed as a function of Vm; the first responses (blue symbols) and the second responses (red symbols). (D) Comparison of the action spectrum of the average light-pulse response at 73.9 mV with SEM bars; naïve hWR (blue, n=6) vs hWR-W163F (red, n=7). Each arrow indicates the action maxima (nm): 449 (blue) and 463 (red) for naïve hWR vs hWR-W163F, respectively. Each line was drawn according to a Gaussian distribution for the light frequency.