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. 2020 Oct 26;6(4):1175–1188. doi: 10.1016/j.bioactmat.2020.10.010

Fig. 3.

Fig. 3

Osteogenic and angiogenic differentiation of PDLSCs on different fibrous membranes. (A,B) ALP activity at day 7 and 14. (C–F) qRT-PCR analysis for the relative mRNA levels of Runx-2 (C), OPN (D), OCN (E) and BSP (F) after cultured for 7, 14 and 21 days. (G–J) Representative images of tubule formation assay of PDLSCs cultured on different fibrous membranes. Scale bars = 400 μm (K–N) Quantitative analysis of number of nodes (K), number of segments (L), number of meshes (M) and the total tube length (N) of the formed tubules. (O–R) qRT-PCR analysis for the relative mRNA levels of angiogenesis-related gene VEGF (O), CD31 (P), SCF (Q) and PLGF (R) after cultured for 7 days. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001.