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. 2020 Oct 15;11:538355. doi: 10.3389/fmicb.2020.538355

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Copyright © 2020 Zhang, Lin, Qin, Huang, Sun, Zhao and Jin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of influenza virus nucleoprotein (NP)-associated factors by immunoprecipitation-mass spectrometry (IP/MS). (A,B) Eluates of duplicate hemagglutinin (HA) immunoprecipitates from HA-NP overexpressed DF-1 cells were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and silver staining. (C–G) DF-1 cells were transfected with three different chaperonin containing TCP1 subunit 5 (CCT5), phenylalanyl-tRNA synthetase, alpha subunit (FARSA), RuvB-like AAA ATPase 1 (RUVBL1), NOP56, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L) small interfering RNAs (siRNAs) as indicated for 24 h. Knockdown efficiency was determined by real-time PCR and Western blot. The data are presented as the means ± SD from three independent experiments (^*p < 0.05; ^**p < 0.01; ^***p < 0.001, by t-test).