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. 2020 Oct 15;11:538355. doi: 10.3389/fmicb.2020.538355

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Copyright © 2020 Zhang, Lin, Qin, Huang, Sun, Zhao and Jin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The loss of CCT5 restricts H5N6 replication in DF-1 cells. (A,B) Viral nucleoprotein (NP)/polymerase acidic protein (PA)/polymerase basic protein 2 (PB2) messenger RNA (mRNA) abundance in DF-1 cells that were transfected with indicated siRNAs or a negative control followed by infection with 0.1 multiplicity of infection (MOI) H5N6-GFP virus. The cell supernatants were collected at 12, 24, and 36 h postinfection and subjected to TCID₅₀ measurement in Madin-Darby canine kidney (MDCK) cells (^*p < 0.05; ^**p < 0.01; ^***p < 0.001, by t-test).