Figure 3.

Chaperonin containing TCP1 subunit 5 is associated with viral nucleoprotein (NP), polymerase basic protein 2 (PB2), and polymerase basic protein 1 (PB1) but not polymerase acidic protein (PA) by coimmunoprecipitation (co-IP) assay. (A,B) Hemagglutinin NP (HA-NP) or HA-PB2, together with CCT5-Flag, were transfected into DF-1 cells for 24 h. Sample lysates were incubated using agarose beads pretreated by HA or Flag antibodies for 2 h at room temperature. The IP eluates were subjected to Western blot analysis. (C–F) DF-1 cells were infected with 0.1 MOI H5N6-GFP for 12 h. Co-IP experiment was performed as described in the Materials and Methods section, and the IP products were subjected to Western blot analysis. Immunoglobulin G (IgG) antibody coprecipitation was used as the control group. The presented results were from three independent experiments.