Figure 5.

Influenza virus infection promotes the expression of CCT5 in DF-1 cells. (A) DF-1 cells were infected with or without 0.1 MOI H5N6-GFP virus for 12, 24, and 36 h, respectively. The mRNA and protein levels of CCT5 and nucleoprotein (NP) were measured by real-time PCR and Western blot analysis. (B–D) DF-1 cells were infected with the indicated dose of H5N6-GFP, H5N1-HM, or H5N1-DW virus for 12 h; the mRNA and protein levels of CCT5 and NP were detected by real-time PCR and Western blot, respectively. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a loading control for protein. Chicken ACTIN gene was used to normalize mRNA expression (^*p < 0.05; ^**p < 0.01; ^***p < 0.001; from three independent experiments by two-tailed Student’s t-test).