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. 2020 Oct 15;11:538355. doi: 10.3389/fmicb.2020.538355

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Copyright © 2020 Zhang, Lin, Qin, Huang, Sun, Zhao and Jin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Silencing CCT5 inhibits influenza virus replication in DF-1 cells. (A) DF-1 cells were transfected with siCCT5#1 or NC for 12 h, and the cell viability was measured by CCK-8. (B) Growth curves of H5N6-WT and H5N6-GFP virus were determined in CCT5 knockdown DF-1 cells. (C,D) Effect of overexpressed or suppressed CCT5 on viral RNA (vRNA) synthesis. DF-1 cells transfected with CCT5-Flag or siCCT5#1 followed by infection with 0.1 MOI H5N6-GFP virus. The levels of nucleoprotein (NP) vRNA, complementary RNA (cRNA), and mRNA were determined by real-time PCR. (E,F) NP protein levels were also monitored by Western blot analysis. GAPDH was used as a loading control and marker. GAPDH was used as a loading control for protein. Chicken ACTIN gene was used to normalize mRNA expression (^*p < 0.05; ^**p < 0.01; ^***p < 0.001; from three independent experiments by t-tests).