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. 2020 Oct 15;11:538355. doi: 10.3389/fmicb.2020.538355

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Copyright © 2020 Zhang, Lin, Qin, Huang, Sun, Zhao and Jin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of CCT5 on nucleoprotein (NP) distribution in DF-1 cells. (A–C) DF-1 cells were transfected with siCCT5#1 or CCT5-Flag plasmid for 24 h and then infected with 0.1 MOI H5N6-GFP. At 3, 6, and 9 h postinfection, cytoplasm, and nucleoplasm were separated for Western blot analysis. LaminB1 was used as a nuclear loading control. GAPDH was used as a cytosolic loading control. (D–F) The DF-1 cells were treated following the same procedure as above and then subjected to stain with CCT5 or NP primary antibody followed by secondary antibodies as described in Materials and Methods section. The localization of CCT5 and viral NP was observed under confocal microscopy.