TABLE 5.
Characteristics of 20 newly developed polymorphic microsatellite markers in P. chienii.
| Locus | Primer sequence (5′-3′) | Repeat motif | Size range (bp) | Annealing temperature (°C) | Na | Ho | He | PIC* | Null | GenBank accession no. |
| P. chienii-17 | F: GGTCGAGTACGTGGTGGTTT R: GCCTGCGCTGTCATAAACTG | (AGG)5 | 158–161 | 57 | 3.000 | 0.145 | 0.257 | 0.237** | 0.2703 (Y) | MT563347 |
| P. chienii-20 | F: TGTGCCAGTACTGCTACTGC R: TGAATGCGTGCGGAAACAAG | (ACC)6 | 183–195 | 57 | 5.000 | 0.209 | 0.585 | 0.517 | −0.0267 (N) | MT563350 |
| P. chienii-25 | F: GATGCCGCTGGTTTCAATCC R: GCCGTACCGATTGGGATCAT | (GGA)8 | 198–214 | 57 | 5.000 | 0.309 | 0.397 | 0.366* | 0.315 (Y) | MT563351 |
| P. chienii-28 | F: GAGTGGGAGACGAAGAGTGC R: CGAAGTGGGCTGCAACAATG | (CTC)8 | 261–266 | 57 | 6.000 | 0.600 | 0.670 | 0.626 | 0.0196 (N) | MT563352 |
| P. chienii-29 | F: AGCTGCAAGGCTACACAGAG R: CAATCCCGGGCCTGTTAGAA | (GAA)5 | 235–239 | 57 | 5.000 | 0.318 | 0.563 | 0.500** | −0.1413 (N) | MT563353 |
| P. chienii-36 | F: GGGCCATCCTCTTCCTCAAC R: CTCGACACTGCTCCACATCT | (TCC)8 | 240–247 | 57 | 3.000 | 0.055 | 0.129 | 0.125** | 0.0299 (N) | MT563358 |
| P. chienii-75 | F: CGCTCCAACGAATCCAACC R: TAATGCCATCCGCACAACC | (CAGAAG)5 | 241–277 | 57 | 14.000 | 0.743 | 0.844 | 0.826 | −0.1586 (N) | MT563368 |
| P. chienii-86 | F: GAATTTGAAGCACGGCCTCA R: GAGTGCCCTGCTTTCTGGAT | (GGCACC)5 | 242–277 | 57 | 7.000 | 0.156 | 0.595 | 0.549** | 0.3533 (Y) | MT563369 |
| P. chienii-134 | F: ACGCCACGTTAGGACACAAT R: CCTAGATCAAGAGCGGCCTG | (CTT)6 | 238–319 | 57 | 6.000 | 0.227 | 0.389 | 0.364 | 0.0983 (N) | MT563374 |
| P. chienii-141 | F: CTGTCAACAAGCGGCTTTCC R: AGAGCCGGGGGAAAATTGAG | (CGG)7 | 232–319 | 57 | 8.000 | 0.473 | 0.694 | 0.640** | 0.0296 (N) | MT563377 |
| P. chienii-152 | F: CCCATCTGAACCCACGCTAA R: AAAGCGCTCATGCCCAAAAC | (GGC)7 | 239–300 | 57 | 16.000 | 1.000 | 0.887 | 0.877** | −0.2134 (N) | MT563378 |
| P. chienii-162 | F: ACCTATCACCTCCTCGACCC R: CCGTTCCATCACTGTGGACA | (CCACCG)6 | 203–229 | 55 | 13.000 | 0.573 | 0.807 | 0.784** | 0.3871 (Y) | MT563379 |
| P. chienii-198 | F: GAGGGATACAGAAGCACAG R: TATGACAAACCCAAACGAG | (ATA)5 | 276–288 | 56 | 2.000 | 0.591 | 0.416 | 0.330** | 0 (N) | MT563329 |
| P. chienii-214 | F: GACAACGGCAAAGGAGGAAT R: GCGATAGCCACCAAAGACAT | (ATA)6 | 319–322 | 58 | 3.000 | 0.000 | 0.329 | 0.290** | 0 (N) | MT563334 |
| P. chienii-216 | F: TGCGGTTCAGTAACAGTCCTTC R: TCCCCCACCTCTTCCCAG | (CTCCTG)5 | 439–452 | 58 | 6.000 | 0.657 | 0.513 | 0.408 | −0.314 (N) | MT563335 |
| P. chienii-233 | F: TGTGTGAAAGGACAAGGCGT R: GCACCCTATTCACCCGAGAT | (ATGCAG)7 | 225–259 | 56 | 5.000 | 0.318 | 0.474 | 0.431 | −0.0267 (N) | MT563337 |
| P. chienii-267 | F: CCCCTCATTGACAGGTTC R: AAGATAGTCGGGACACCAAG | (CTG)5 | 309–320 | 56 | 6.000 | 0.036 | 0.336 | 0.306** | 0.3232 (Y) | MT563381 |
| P. chienii-288 | F: CACGCCCACCATAGTTGT R: GGAGGAAGATGTCGTTGAAG | (AAGG)5 | 263–270 | 58 | 3.000 | 0.045 | 0.523 | 0.449** | 0.2884 (Y) | MT563386 |
| P. chienii-341 | F: GACCTCTTACCAGCTGCGAG R: ACCACCGGTTTCAGTTTCGT | (CCT)11 | 208–227 | 62 | 9.000 | 0.200 | 0.765 | 0.726** | 0 (N) | MT563390 |
| P. chienii-358 | F: TAAGTGGCTGCTGCATCACA R: TACAGCAGCAGCAGAGCTTT | (TCC)5 | 249-251 | 58 | 3.000 | 0.318 | 0.360 | 0.302 | 0.1828 (N) | MT563392 |
Locus, locus name; Na, number of alleles; Ho, observed heterozygosity; He, expected heterozygosity; PIC, Polymorphism information content (*P < 0.05, significantly departures from Hardy–Weinberg equilibrium; **P < 0.01, extremely significantly departures from Hardy–Weinberg equilibrium); Null, the frequency of null allele (Y represents that the SSR locus may have a null allele, N represents that the SSR locus may not have null allele).