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. 2020 Oct 15;8:566617. doi: 10.3389/fcell.2020.566617

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Copyright © 2020 Kubo, Ogawa, Uehara and Katakura.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of treatment with polyphenols on the proliferation of cells and expression of marker proteins in the skin sections with hair follicles. After 35 days of treatment, immunohistological analysis of skin sections was performed using anti-Ki-67 antibody and DAPI staining (A, Control; B, Resveratrol; C, Fisetin; arrow head; Ki67⁺ cells). (D) Immunohistological analysis of skin sections treated with fisetin at early anagen and active anagen were performed using anti-Tert antibody, anti-active-β-catenin antibody and anti-CD34 antibody (DP, dermal papilla; Mx, matrix; EP, epithelium). Immunohistological analysis of non-treated skin sectons were also shown (Control).