FIGURE 4.

¹³CO₂ pulse-labeling of wild-type, quadruple and quintuple rice cross lines. Fully expanded leaves at mid-tillering stage were pulse-labeled with ¹³CO₂ (300 ppm) for 60 s under steady state photosynthetic conditions. Isotopomers of malate, aspartate, 3PGA, PEP, and citrate + isocitrate were measured in extracts from pulse-labeled (60 s) and non-labeled (0 s) leaves by LC-MS/MS, and ¹³C enrichment (%) was calculated after correction for natural abundance. Values are means ± SE (n = 2–4). The original data are presented in Supplementary Dataset A. Different letters within groups indicated those values that are statistically different based on a one-way ANOVA, P-value < 0.05.