Figure 3.

Cells cultured in growing media undergo cell death in response to the autophagy inhibitory compounds, 3-methyladenine, SBI-0206965, Bafilomycin A₁ or Chloroquine. MEFs were treated with the drugs at the concentrations stated in the panel. (A) After 24 h, cell viability was measured by the cellular ability to reduce AB reagent. Bar value is the mean ± SEM (n = 3). Student’s t-test *P < 0.01, **P < 0.005 and ***P < 0.001. (B) After 48 h, the percentage of propidium iodide (PI)-positive cells (dead cells) was determined by flow cytometry. Bar value is the mean ± SEM (n = 3). Student’s t-test **P < 0.005 and ***P < 0.001.