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. 2020 Oct 7;23(11):101653. doi: 10.1016/j.isci.2020.101653

Figure 2.

Figure 2

Effect of the Flow Rate and the Shear Rate on the Cell Growth and Viability

Viable cell density and viability in batch cultures performed with (A) pump tubes GORE STA-PURE or PharMed, (B) ATF at different flow rates and (C) TFF at different flow rates; error bars indicate duplicate runs. Effect of flow rate and average shear rate on the cell growth in ATF and TFF systems operated at different flow rates; growth rate, average of the values at days 2 and 3, represented as function of the flow rate (D-left) and the shear rate (D-right) calculated by Equations 1 and S15 – error bars give the variation in repeated experiments listed in Material and Method. Effect of flow rate and shear rate on the average growth rate ratio (growth rate ratio = growth rate/control growth rate, where control is absence of flow 'No shear') by regression analysis (red lines) for ATF and TFF (E), and for TFF only (F) showing a strong correlation between the growth rate ratio and the flow rate or the shear rate in TFF system, as indicated by the adjusted R2 value.