Figure 3.

Cells Display Distinct Gene Expression Responses to Shear Stress

(A) PCA of Log₂-normalized gene expression in control, ATF cultivated for 3 days (ATF_D3), and TFF cultivated for 2 (TFF_D2) or 3 (TFF_D3) days.

(B) PCA with respect to different flow rates.

(C) Differentially expressed genes (cyan, FDR <0.05) and non-differentially expressed genes (red) as function of Log₂-fold change differences, between ATF and TFF.

(D) Enriched biological processes (FDR <0.005) as function of biological process change, for the comparison between high and low shear stress. Dot sizes are proportional to the significance.

(E) Biological processes exclusively up-regulated (FDR <0.001) in TFF and ATF. Colors indicate up-regulation strength, from least (yellow) to most (red) enriched terms.

(F) Venn diagram illustrating genes with TPM value > 1 for low, high, and control shear stress conditions – Data in Tables S1, S2, S3, and S4