FIGURE 2.
Analysis of affinity of antibodies by biolayer interferometry (BLITz). BLItz shows the binding affinities of immobilized monoclonal antibodies toward different constructs of recombinant human tau (full length or repeat domain) and mouse tau. A, Graphical BLItz sensogram (raw data) shows the binding affinities of immobilized antibodies 2B10 (2677 nM) and 6H1 (866.7 nM) toward TauRDΔK (green), TauFL (red), and mTau (black). The sensograms show ka and kd values of different tau construct toward antibodies. KD values are calculated from the obtained ka and kd values. B, Table showing affinities (ka, kd, and KD) of antibodies to tau protein based on analyzed sensogram. Abbreviations; RD, repeat domain tau; ka, association rate constant; kd, dissociation rate constant; KD, dissociation constant/binding affinity