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Evaluation of the clinical performance of direct RT-qPCR protocol compared to the standard protocol as reference.
| Validation stage (Cq <40) | Evaluation of problematic samples (Cq ≥40) | |
|---|---|---|
| True positive | 27 | 31 |
| True negative | 103 | 16 |
| False positive | 0 | 2 |
| False negative | 0 | 1 |
| Total | 130 | 50 |
| Classification concordance | 100% | 94% |
Concordance for each, the validation and evaluation of problematic samples are shown.
