Table 3.
Antibodies Used for Multiparametric Flow Cytometric Analysis
| Antibody (Clone) | Dilution | Manufacturer |
|---|---|---|
| Anti-CCR7-BV421 (150503) | 1:33.33 | BD Biosciences, Germany |
| Anti-CD38-BUV737 (HB7) | 1:200 | BD Biosciences, Germany |
| Anti-ICOS-BV711 (DX29) | 1:100 | BD Biosciences, Germany |
| Anti-PD-1-BV786 (EH12.1) | 1:33.33 | BD Biosciences, Germany |
| Anti-CD4-FITC (RPA-T4) | 1:33.33 | BD Biosciences, Germany |
| Anti-CD45RA-PE (HI100) | 1:200 | BD Biosciences, Germany |
| Anti-CD134(OX40)-PE-Cy7 (ACT-35) | 1:33.33 | BD Biosciences, Germany |
| Anti-CXCR5-APC (J252D4) | 1:200 | BioLegend, United Kingdom |
| Anti-fixable viability dye-eF780 | 1:5000 | eBioscience, Germany |
| Anti-CD14-eF780 (61D3) | 1:100 | eBioscience, Germany |
| Anti-CD19-eF780 (HIB19) | 1:100 | eBioscience, Germany |
| Anti-CD4-BB515 (SK3) | 1:200 | BD Biosciences, Germany |
| Anti-IL-17-PE (eBio46DEC17) | 1:50 | eBioscience, Germany |
| Anti-CD4-BV510 (SK3) | 1:10 | BD Biosciences, Germany |
| Anti-PD-1-PerCPeFlour710 (eBioJ105) | 1:20 | eBioscience, Germany |
| Anti-CXCR5-BV421(J252D4) | 1:20 | BioLegend, United Kingdom |