FIG 3.

False-positive read extraction depends on stringency cutoffs, but only BBmap results improve with read length. Origin of reads extracted by SortMeRNA and BBmap at different parameters (horizontal axes) from simulated metagenome of 100-bp paired-end (PE) reads (above) or 150-bp PE reads (below), showing the proportion of total originating from actual SSU rRNA features (left) and the origin of non-SSU rRNA-extracted reads (right). Horizontal lines indicate the total actual SSU rRNA origin reads in each library (20,213 and 13,829, respectively).