FIG 4.

Mapper and cutoff settings influence SSU rRNA read extraction from Tara Oceans metagenome libraries but yield highly similar assembly ratios. (a) Fraction of reads extracted by both tools at different BBmap minimum identities (id.) (horizontal) and SortMeRNA E-value cutoffs (vertical). (b) Reads assembled by SPAdes versus total reads extracted for different parameter settings (colors) of SortMeRNA (top) and BBmap (bottom), overlaid with linear regression lines and 1:1 line for reference (gray).