Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 15;8:594226. doi: 10.3389/fcell.2020.594226

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Zhao, Ye, Su and Garg.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Robust proliferation of human iPSC-CMs is promoted by WNT signaling activation. (A) Typical morphology of human iPSC-CMs stained with antibodies against cardiac troponin T (TNNT2, green) and α-actinin (red). Nuclei were counterstained with DAPI (blue). (B) Zoom-in sarcomere structure of human iPSC-CMs with intercalated TNNT2 (green) and α-actinin (red). (C,D) Robust proliferation of human iPSC-CMs from Day 1 (D1,C) to D7 (D) in the presence of 2 μM of a WNT activator (CHIR99021). (E,F) Dramatic increase of dividing CMs is propelled by CHIR99021. Cells were stained with Ki67 (green) and TNNT2 (red). Nuclei were counterstained with DAPI (blue). Double positive cells (indicated by white arrows) are dividing cardiomyocytes. The percentage of Ki67⁺ TNNT2⁺ cells is increased in CHIR99021-treated iPSC-CMs (F) compared to the controls (E). Scale bars: 10 μm (B); 100 μm (A,E,F); 200 μm (C,D).