Fig. 4.
Biosensor response of constructed LysG-based biosensors in C. glutamicum. (A) Dose-response plot for LysG-based sensor constructs based on the native regulatory circuit (pSenLysG) and on the unified sensor design (pSCCg-LysG-S/M/W). All different cultivations were supplemented with eight different inducer concentrations ranging from 125 to 10,000 μM dipeptide His-Ala (externally supplemented). The respective fluorescence response was plotted as fold-change in specific eYFP fluorescence. Error bars represent standard deviations calculated from three biological replicates. (B) FACS experiments with C. glutamicum ΔlysEG strains carrying pSenLysG or pSCCg-LysG-S/M/W in the presence of externally supplemented 0 μM His-Ala (blue), 1250 μM His-Ala (pink) and 2500 μM His-Ala (green). In each case 95,000 representative single cells were analyzed. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)