Fig. 5.
Ligand spectrum of LysG-based biosensors in C. glutamicum. (A) Dose-response plot for reference pSenLysG and (B) sensor construct pSCCg-LysG-S in the presence of externally supplemented inducers His-Ala (blue), Lys-Ala (pink) and Arg-Ala (green). Cultivations were supplemented with eight different inducer concentrations ranging from 125 μM −10,000 μM (externally supplemented). The respective fluorescence response was plotted as fold-change in specific eYFP fluorescence. Error bars represent standard deviations calculated from three biological replicates. FACS experiments with C. glutamicum ΔlysEG strains carrying (C) pSenLysG or (D) pSCCg-LysG-S in the presence of 5000 μM His-Ala (blue), Lys-Ala (pink) and Arg-Ala (green) (externally supplemented). In each case 95,000 representative single cells were analyzed. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)