Table 1. Development and genome editing rates of embryos electroporated nucleases, including tracrRNA-ATTO550 at different concentrations.

Conc. of tracrRNA-ATTO550 (μM)	Conc. of tracrRNA (μM)	No. embryos electroporated	No. (%) of embryos survived after electroporation	No. (%) of embryos with fluorescence	No. (%) of embryos developed to 2-cell stage and transferred	No. (%) of offspring	No. (%) of knockout offspring
0	15	35	35 (100.0 ± 0.0)	-	32 (90.0 ± 12.2) a	15 (50.7 ± 15.1)	7 (46.7 ± 8.2)
7.5	7.5	29	29 (100.0 ± 0.0)	29 (100.0 ± 0.0)	26 (91.0 ± 11.0) a	11 (43.7 ± 16.3)	10 (91.7 ± 10.2)
15	0	84	82 (97.6 ± 6.5)	82 (100.0 ± 0.0)	20 (21.7 ± 16.3) b	4 (10.0 ± 3.67)	1 (16.7 ± 40.8)

The pulse length of the poring pulse was set to 3.5 msec. Percentages were showed as the mean ± SEM. a vs. b. Significant differences at P < 0.05.