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Localization of H4K20me1 through preimplantation development. Embryos were collected at the one-cell [15 h post-insemination (hpi), n = 9), two-cell (34 hpi, n = 11), four-cell (48 hpi, n = 11), eight-cell (56 hpi, n = 11), morula (72 hpi, n = 12), and blastocyst (96 hpi, n = 11) stages. Embryos were stained with anti-H4K20me1 antibody. H4K20me1 was present at all stages of preimplantation development. Scale bar, 10 μm.
