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. 2020 May 6;66(5):411–419. doi: 10.1262/jrd.2020-036

Fig. 4.

Fig. 4.

Cell-cycle progression and DNA damage in embryos lacking H4K20me1. (A) Incorporation of EdU in embryos treated with (n = 10) or without (n = 9) UNC0379 at 9 h post-insemination (hpi) and in embryos overexpressing H4K20WT (n = 16) and H4K20M (n = 16) at 22 hpi. Scale bar, 25 μm. (B) Upper photo shows the presence of two pronuclei at 24 hpi in a one-cell embryo treated with UNC0379 for 18 h (6–24 hpi, n = 20) and the lower photo shows the presence of two nuclei at 48 hpi (n = 8) in a two-cell embryo overexpressing H4K20M. Scale bar, 10 μm. (C) The number of γH2AX foci was larger in embryos treated with UNC0379 (n = 27) than in non-treated embryos (n = 24) at 11 hpi; at 15 hpi, the γH2AX foci almost disappeared in non-treated embryos (n = 27) but increased in embryos treated with UNC0379 (n = 23). Details show larger magnification of the white boxed regions. Scale bars, 16 μm. (D) There was no difference in the number of γH2AX foci at 15 hpi between embryos overexpressing H4K20WT (n = 19) and H4K20M (n = 21). However, larger number of γH2AX foci was observed at 34 hpi in embryos overexpressing H4K20M (n = 24) than in embryos overexpressing H4K20WT (n = 23). Details show larger magnification of the white boxed regions. Scale bar, 16 μm.