Table 1. In vitro developmental potential of oocytes collected via surgical operation with anesthesia after in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI).
Exp. | Collection method | No. of used mice * | No. of used oocytes | No. of oocytes surviving after ICSI |
No. (%) ** of oocytes with 2PN formed |
No. of embryos developed to |
|||
---|---|---|---|---|---|---|---|---|---|
2 cell | 4–8 cell | Morula | Blastocyst (%) ** | ||||||
IVF | Control | 17 | 376 | - | 353 (93.9) a | 348 | 335 | 325 | 319 (90.4) a |
Operation | 42 | 1022 | - | 963 (94.2) a | 939 | 894 | 861 | 852 (88.5) a | |
ICSI | Control | 20 | 286 | 214 | 198 (92.5) b | 192 | 165 | 144 | 130 (65.7) b |
Operation | 17 | 294 | 218 | 204 (93.6) b | 195 | 175 | 160 | 148 (72.5) b |
Some experimental groups were not performed with control at the same time. PN: Pronuclei. * The data of oocytes collected from these mice were used not only in this table but also in Table 2. ** Identical letters indicate no significant differences by Pearson’s chi-squared test (P > 0.01).