Table 3. Number and normal rate of Second-oocytes collected from the reused mice.
Recovery period after surgery |
No. of used mice (oviducts) |
No. (%) of mice with COCs contained in oviducts |
No. of oocytes |
Average no. of normal oocytes |
||||
---|---|---|---|---|---|---|---|---|
Without COCs | Only 1 oviduct | Both oviducts | Total | Normal oocytes (%) [average] * | Per mouse with COCs | Per oviduct contained COCs | ||
Control | 29 (58) | 0 | 0 | 29 (100.0) | 1150 | 910 (79.1) [31.4] a | 31.4 | 15.7 |
10 days | 46 (92) | 23 (50.0) | 19 (41.3) | 4 (8.7) | 550 | 476 (86.5) [10.3] b | 20.7 | 17.6 |
20 days | 53 (106) | 25 (47.2) | 24 (45.3) | 5 (9.4) | 614 | 567 (92.3) [10.7] b | 19.6 | 16.7 |
30 days | 65 (130) | 34 (52.3) | 23 (35.4) | 8 (12.3) | 681 | 581 (85.3) [8.9] b | 18.7 | 16.1 |
COCs: Cumulus oocytes complexes. * Different letters indicate significant differences by t-test (P < 0.01).