. 2020 Jun 6;66(5):427–433. doi: 10.1262/jrd.2020-059

Table 6. Blastocyst and full-term development of in vitro fertilization (IVF) embryos derived from germinal vesicle (GV)-stage oocytes of reused mice.

Recovery period after surgery	No. of used mice	No. (average) * of GV oocytes	No. (average) * [%] of MII oocytes after IVM	No. of used oocytes	No. (%) ** of oocytes with 2PN formed	No. of embryos developed to				No. of transferred embryos (no. of recipients)	No. (%) ** [min–max] of offspring
Recovery period after surgery	No. of used mice	No. (average) * of GV oocytes	No. (average) * [%] of MII oocytes after IVM	No. of used oocytes	No. (%) ** of oocytes with 2PN formed	2 cell	4–8 cell	Morula	Blastocyst (%) **	No. of transferred embryos (no. of recipients)	No. (%) ** [min–max] of offspring
Control	16	493 (30.8) ^a	381 (23.8) ^a [77.3]	264	156 (59.1)	143	111	87	69 (44.2) ^a	–	–
Control	16	493 (30.8) ^a	381 (23.8) ^a [77.3]	66	48 (72.7)	44	–	–	–	44 (4)	13 (29.5) ^a [22.2–33.3]
10 days	12	327 (27.3) ^a	203 (16.9) ^a [62.1]	155	95 (61.3)	89	64	43	40 (42.1) ^a	–	–
10 days	12	327 (27.3) ^a	203 (16.9) ^a [62.1]	47	31 (66.0)	30	–	–	–	30 (2)	13 (43.3) ^a [31.3–57.1]
20 days	14	425 (30.4) ^a	302 (21.6) ^a [71.1]	281	167 (59.4) ^a	155	116	94	78 (46.7) ^a	–	–
20 days	14	425 (30.4) ^a	302 (21.6) ^a [71.1]	19	16 (84.2) ^b	16	-	–	–	16 (1)	5 (31.3) ^a [31.3]
30 days	27	733 (27.1) ^a	552 (20.4) ^a [75.3]	426	267 (62.7)	240	170	115	97 (36.3) ^a	–	–
30 days	27	733 (27.1) ^a	552 (20.4) ^a [75.3]	88	66 (75.0) ^b	63	–	–	–	63 (4)	18 (28.6) ^a [15.0–63.6]

Some experimental groups were not performed with control at the same time. PN: Pronuclei. * Identical letters indicate no significant differences by t-test (P > 0.01). ** Identical letters indicate no significant differences by Pearson’s Chi-square test (P > 0.01).